Phospho mtor ser2448

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August undefined, 2024

WebInvitrogen Anti-Phospho-mTOR (Ser2448) Polyclonal, Catalog # 44-1125G. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts … WebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation …

Phospho-mTOR (Ser2448) Antibody from Cell Signaling Technology …

WebPhosphoPair mTOR (Ser2448) Antibody Set. Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells treated with 10 µM rapamycin (Rap) for 2 hours or with … WebPhospho-mTOR (S2448) and Total mTOR ELISA Kit (ab279869) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated mTOR protein in your experimental model system, you can verify pathway activation in your cell lysates. manotick animal hospital

Phospho-mTOR (Ser2448) Antibody Cell Signaling …

WebA synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR. Formulation. 1X PBS, 0.09% NaN3, 0.2% BSA. Isotype. Rabbit IgGk. Preparation. Protein A+G. Recommended Usage. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. Web斯氏毛滴虫pcr检测试剂盒厂家上海联祖生物相关产品:28s rrnp检测试剂盒实验流程1.实验前标准品、试剂及样本的准... Webresults in elevated mTOR activity in vitro and in cells (18, 20). In this study, we demonstrate that mTOR phosphorylation at Ser-2448 is blocked by rapamycin, and this effect is inde-pendent of the AKT activation status, which suggests that Ser-2448 phosphorylation is catalyzed by a protein kinase other than AKT. We show that Ser-2448 is ... kothrade plumbing

Phospho-mTOR (Ser2448) (49F9) Rabbit mAb - Cell …

Phospho-mTOR (Ser2448) Monoclonal Antibody …

WebProduct Description This peptide is used to specifically block #2971 Phospho-mTOR (Ser2448) Antibody reactivity. Quality Control The quality of the peptide was evaluated by … WebJan 15, 2009 · mTOR phosphorylated at S2448 can be found in a complex with both, raptor and rictor. Total lysates of logarithmically growing HEK293 cells were prepared and used for immunoprecipitation of endogenous raptor or rictor. manotick breakfast restaurantsWebApr 10, 2024 · RayBio® Human and Mouse Phospho-mTOR (Ser2448) ELISA Kit. This assay semi-quantitatively measures mTOR phosphorylated at Serine-2448 in cell lysate samples. MANUAL / SDS $521.00 Contact Us for Bulk Request Skip to the end of the images gallery Skip to the beginning of the images gallery Product Description Specifications Risk-Free … manotick accounting

"WebWe show that Ser-2448 is directly phosphorylated byp70S6 kinase in vitro and that expression of a rapamycin-resistantp70S6 kinase in cells maintains Ser-2448 phosphorylation in the presence ofrapamycin. Furthermore, changes in amino acid availability, which affectsp70S6 kinase activity, also modulates Ser-2448 phosphorylation. " - Phospho mtor ser2448

Phospho mtor ser2448

Human and Mouse Phospho-mTOR (S2448) ELISA RayBiotech

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 213 publications. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and … WebmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by …

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WebJul 8, 2005 · Previous studies have shown that mTOR is a direct substrate for the AKT kinase and identified Ser-2448 as the AKT target site in mTOR. In this study, we … WebPrimary antibodies for immunohistochemistry (IHC) included anti-mTOR, anti-phospho-mTOR Ser2448, anti-S6 ribosomal protein, anti-Beclin1 (all from Abcam, Cambridge, UK) …

WebThe mTOR subunit of mTORC1 and 2 is phosphorylated at Ser2448 in a PI3/Akt- signaling-dependent manner; this post-translational modification is associated with increased … WebPolyclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1872 publications. Validated for Western Blotting, Simple Western™. Available in 2 sizes. Highly specific and …

WebApr 5, 2024 · Several upstream signals, including ERK, converge on the phosphorylation and inhibition of Tuberous Sclerosis Complex (TSC) to activate mTORC1 [20, 21]. mTOR functions as the catalytic subunit of two individual complexes, known as … WebJan 9, 2024 · Both mTORC1 and mTORC2 are regulated by phosphorylation, complex formation and localization. So far, the role of p70S6K-mediated mTOR S2448 phosphorylation has not been investigated in detail. Here, we report that endogenous mTOR phosphorylated at S2448 binds to both, raptor and rictor.

Webthe complex. Phosphorylation of mTOR at Ser2448 is carried out directly by AKT kinase as well as p70S6 kinase acting as a feedback signal. Phosphorylation at this site is a biomarker for the activation state of the PI-3 kinase pathway as well as the activation status of mTOR. Activation of mTOR leads to phosphorylation of PRAS40,

WebAug 24, 2005 · Anti-mTOR (phospho S2448) antibody [EPR426 (2)] (ab109268) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by … manotick areaWebAnti-phospho-mTOR (Ser2448) Antibody Upstate®, from rabbit; Synonyms: FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1; find … manotick classic boat clubWebmTOR抑制剂的筛选方法. 分析：OD值越小，mTOR的Ser2448磷酸化程度越低，抑制剂作用越强。. （2）荧光共振能量转移（FRET）. 荧光共振能量转移是指两个荧光发色基团在足够靠近（7~10nm）时，当供体分子吸收一定频率的光子后被激发到更高的电子能态，在该电子回 … manotick beer store hoursWebThe Phospho-mTOR (Ser2448) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2448) Antibody has been validated for the following applications: Western Blot. Phospho-mTOR (Ser2448) Antibody detects … manotick carstarWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, … manotick cafeWebNov 7, 2015 · Antibodies against total phospho-AMPK(Thr172), total phospho-ACC(Ser79), total phospho-p53(Ser15), phospho-Raptor (Ser722/ Ser792), total phospho-mTOR(Ser2448), total phospho-p70S6K(Thr389), total phospho4EBP-1 (Thr 37/46), total phospho-Akt(Ser 473), LC3 I/II β-actinwere obtained fromCell Signaling Technology, … k othrine 2 5 flowWebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, which indicates the mTORC1... manotick camping