Cisplatin ic50 a549
WebOct 1, 2024 · Cisplatin-resistant A549 cells (A549/CisR) were derived from A549 cells by continuous cisplatin treatment. The original parental A549 cells were initially treated … WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for …
Cisplatin ic50 a549
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WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were … WebThe 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment Source publication +4 RNA interferences targeting the Fanconi …
WebJan 3, 2003 · We used the IC50 concentrations for both Gleevec and cisplatin to treat A549 cells in culture, and assessed the viability of the cells by MTT assays at the different time points (Figure 4 ). Gleevec (2 μM) and cisplatin (64 μM) both inhibited the growth of the tumor cells individually after treatment of the cells for 48 hour. WebJul 23, 2015 · The IC50 of cisplatin for A549 and SPC-A1 cells under hypoxia was 3.38-fold and 1.57-fold higher as compared to that under normoxia respectively as showing by the …
WebFeb 3, 2024 · A549 and A549/CDDP cells were treated with 1/2 IC50 of cisplatin and/or PD‐0332991 for 24 h. Then cells were homogenized in RIPA buffer (50 mM Tris–HCl, pH … WebNext, the highest cisplatin IC50 was found in CAFs, and A549 cells had the lowest IC50 value . The ... Cell viability of A549 and HCC827 cells treated with exosomes (25 μg/mL) derived from cisplatin-treated A549-R cells or CAFs. Control exosomes (Exo-control) derived from A549 or HCC827 cells’ own culture mediums. ...
WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug …
WebMar 22, 2024 · The IC50 (drug concentration producing 50% growth inhibition) was calculated with GraphPad Prism Version 5.0 Software. Cell proliferation assay Cell … cult worship definitionWebFor A549, except for II, the other complexes exhibited moderate cytotoxicity. For non-tumorigenic ligeages, the complexes were less toxic than cisplatin for MCF-10A, with emphasis on V, which was inactive. In addition, all complexes were more selective than cisplatin at A2780-cis and for MCF-7, V exhibited selectivity index > 6. east moline freedom festWebMay 11, 2016 · A549 cells. After 156 hrs of monitoring, the diameter of A549 spheroid controls decreased by a total of 141 μM. During that time, spheroids treated with 20 and 40 μM of DXR also decreased, shrinking by −206 μM and −178 μM, respectively, the rate of shrinkage was faster in the treated spheroids compared with the controls. east moline fireworks 2022WebNov 19, 2013 · A549 cells were exposed to cisplatin (CDDP) at an IC50 dose (12 µM) or a range of carboplatin (CBCDA) doses and total intracellular ROS levels were measured … cult worksWebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 ... USA; CRM‐CCL‐185), A549 cisplatin‐resistant (A549/DDP; National Infrastructure of Cell Line Resource, Beijing, China; 1101HUM‐PUMC000519), and human lung bronchial epithelial 16HBE cells (Shanghai Fuheng Biotechnology Co., Ltd. China; … east moline il city ordinancesWebImmunofluorescence results elucidated that cellular ROS level in Cisplatin-induced A549 cells increases in a dose-dependent manner. Both immunofluorescence and flow cytometry results revealed that the apoptotic rate of A549 cells increases with the elevation of Cisplatin dose. east moline floristcultwo show live